Application

Research CategoryApoptosis & Cancer

This Anti-BAG-1 Antibody, clone 3.10G3E2 is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and ELISA for the detection of BAG-1.

Western Blot Analysis: A representative lot of this antibody detected BAG-1 in MCF-7 cell lysate (Brimmell, M., et al., (1999). British Journal of Cancer 81(5):1042–1051).

Western Blot Analysis: A representative lot of this antibody detected BAG-1 in colonic epithelial cell lysate (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).

Western Blot Analysis: A representative lot of this antibody detected BAG-1(L, M, S) in BAG-1(L, M, S) overexpressed MCF-7 cell lysates.

Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections (Cutress, R. I., et al., (2003). Oncogene. 22:4973–4982).

Immunohistochemistry Analysis: A representative lot of this antibody detected BAG-1 in breast cancer tissue sections.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from endogenouse MCF-7 cell lysates.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated BAG-1 from Carcinoma-derived HT29 and transformed adenoma PC/AA/C1/SB10 cell lysates (Arhel, N. J., et al., (2003). Int. J. Cancer: 106, 364–371).

Interaction Assay/ELISA: A representative lot of this antibody detected BAG-1 by a BAG-1:HSC70 interaction/ELISA assay (Sharp, A., et al., (2009). FEBS Letters 583:3405–3411).

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BAG family molecular chaperone regulator 1 (BAG-1), also known as Bcl-2-associated athanogene 1, blocks a step in a pathway leading to apoptosis or programmed cell death. BAG-1 enhances the anti-apoptotic effects of BCL2 representing a link between growth factor receptors and anti-apoptotic mechanisms. BAG-1 is ubiquitously expressed throughout most tissues, except the liver, colon, breast, and uterine myometrium.

Immunogen

GST-tagged recombinant protein corresponding to Human BAG-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BAG-1 in 10 µg of HeLa cell lysate.

Specificity

This antibody detects 3 observed isoforms: BAG-1(S) at ~38 kDa, BAG-1 (M) at ~45 kDa & BAG-1 (L) at ~52 kDa.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~38, 45, & 52 kDa observed.
This antibody detects 3 observed isoforms at ~38 kDa (S), ~45 kDa (M) & ~52 kDa (L).